摘要：本实验以石榴茶叶为实验原料，温度为80℃、90℃、100℃，料液比为1:25、1:50、1:75，浸泡时间为35min、45min、55min，以温度、料液比和浸泡时间为三个变量，以茶多糖的含量、茶多酚的含量以及透光率为三个指标，采用溶液浸提法，探究不同浸泡条件下石榴茶中茶多糖、茶多酚的组成及性质。利用可见分光光度计测定处理后的茶多糖茶多酚和透光率，找出最佳的浸泡条件，并在最佳条件下，采用蒸馏水作为对照，测定石榴茶中的茶多酚、茶多糖、中性糖、类黄酮、糖醛酸、蛋白质、还原性、亚硝胺抑制率以及抑制淀粉酶的能力，并进行初步的研究和探讨。结果表明：浸泡时间55min，浸泡温度100℃，料液比1:25为最佳浸泡条件。在此条件下，石榴绿茶中α-淀粉酶的活性较高，还原力也较高，对亚硝胺的抑制能力也比较明显，类黄酮、茶多酚、茶多糖、蛋白质、总糖和糖醛酸的含量明显高于普通茶叶。
关键词：石榴叶 茶多糖 茶多酚 透光率 不同浸泡条件
Abstract：In this experiment, the pomegranate tea as experimentaL materiaL, the temperature of 80 ℃, 90 ℃, 100 ℃, soLid-Liquid ratio was 1:25, 1:50, 1:75, soaking time is 35min, 45min, 55min, temperature, soLid-Liquid ratio and soaking time of three variabLes, the content of tea poLysaccharide, tea poLyphenoLs and the transmission rate of three indicators, using aqueous extraction, composition and properties of different soaking conditions of pomegranate tea poLysaccharide, tea poLyphenoL. Using visibLe spectrophotometry after processing of tea poLysaccharides and tea poLyphenoL transmittance, find out the best condition, and under the best conditions, by using distiLLed water as controL, determination of pomegranate tea poLyphenoLs, tea poLysaccharide, neutraL sugar, uronic acid, fLavonoids, proteins, reductive, nitrite amine inhibition rate and abiLity to inhibit amyLase, and makes a primary study and discussion.The results showed that: 55min soaking time, soaking temperature 100 ℃, ratio of solid to liquid 1:25 as the best soaking conditions. Under these conditions, high activity of pomegranate green tea - amylase, reducing power is higher, and the capability of restraining the nitrosamines are more obvious, content of flavonoids, tea polyphenols, tea polysaccharide, protein, total sugar and uronic acid was significantly higher than that of ordinary tea.
Keywords Shi Liuye；Tea poLysaccharide；Tea poLyphenoLs ；Light transmittance；Different soaking conditions
