摘要:利用黑曲霉08013菌株发酵生产内切型菊粉酶，并对发酵培养基的配方进行优化。首先测定菌种产酶的初始酶活为1.65U/mL。然后进行单因素试验，通过与初始酶活的比较确定发酵培养基的最佳单因素分别为碳源乳糖、有机氮源牛肉膏和无机盐硫酸铜，最佳浓度分别为乳糖(9%)、牛肉膏(4%)和硫酸铜(0.7%)。通过正交试验确定生产内切型菊粉酶的发酵培养基的最佳配方为：乳糖(11%)、牛肉膏(4%)、硫酸铜(0.5%)，装瓶量80mL/250mL，30°C，180r/min，发酵4d。此时黑曲霉08013所产内切型菊粉酶的酶活为9.72U/mL，比初始酶活提高了489.09%。
关键词 内切型菊粉酶；黑曲霉；发酵培养基；酶活
Abstract:Producing the endo-inulinase by strain 08013 of aspergillus niger and optimizing the formula of fermentation medium. Firstly, the initial activity of enzyme production by strains reached 1.65U/mL. Then making the single factor experiments. The composition of the best single factor in fermentation medium was carbon source of lactose, organic nitrogen of beef extract, inorganic salt of copper sulfate and the best concentration was lactose(9%), beef extract(4%), cooper sulfate(0.7%)by comparison with the initial enzyme activity. Determination of the best formula in fermentation medium was lactose(11%), beef extract(4%) and copper sulfate(0.5%), medium volume 80mL/250mL, temperature 30°C, and shaking at 180r/min for 4d by the orthogonal experiments. The activity of endo-inulinase production by strain 08013 of aspergillus niger was 9.72U/mL, which increased by 489.09% than the initial activity.
Keywords endo-inulinase aspergillus niger fermentation medium enzyme activity
