摘要:以生姜为原料，通过单因素和正交试验对生姜多糖溶液进行脱蛋白、脱色、超滤等处理的最佳工艺条件进行了探讨。采用多种脱蛋白方法，以及树脂脱色处理。结果表明木瓜蛋白酶-Sevag脱蛋白效果最好，蛋白清除率可达89.92%。通过正交和单因素实验对生姜多糖纯化工艺中大孔树脂的应用条件进行探讨，确定了最佳纯化的工艺和条件。结果表明：D101大孔树脂对生姜多糖的吸附能力最强，树脂的用量为25 g，吸附速率为0.5 mL/min。对生姜多糖提取液进行超滤实验，结果表明：以糖截留率作为衡量指标，其最佳工艺是采用10×103的超滤膜，最佳超滤时间为90 s。对于生姜多糖的抗氧化性试验，结果表明：在一定的浓度范围内，生姜多糖对于羟基自由基和DPPH的清除效率随其浓度的增加而增强，其IC50分别为10.93 μg/mL和11.36 μg/mL。
关键词：生姜多糖；脱色；分离；纯化；树脂；超滤
Abstract:The raw materials used in this experiment ginger, the ginger polysaccharide solution through single factor and orthogonal experiments, the optimum conditions were de-protein, bleaching, ultrafiltration treatment. Anthrone - sulfuric acid method to draw a glucose standard curve. Using a variety of methods of removing protein, as well as resin bleaching treatment. The results showed that the papain-Sevag removing protein the best protein clearance rate of up to 89.92%. Ginger extract pigment purified by orthogonal and single factor experiment macroporous resin in the amount of ginger polysaccharide purification process, the type of resin, the material liquid concentration, adsorption velocity impact factors are discussed to determine the best purification process and conditions. The results showed that: D101 macroporous resin adsorption capacity of ginger polysaccharide is the strongest, the amount of resin to 25 g, the adsorption rate of 0.5 mL / min. Ginger polysaccharide extract ultrafiltration experiments, the results showed that: sugar retention rate as measured by indicators, the best process is the use of 10 × 103 of the ultrafiltration membrane, the best super-The filtration time of 90 s. Ginger polysaccharide oxidation resistance test results showed that: in a certain concentration range, ginger polysaccharide with its increasing concentration of hydroxyl radicals and DPPH scavenging efficiency is enhanced, and its IC50 were 10.93 μg/mL and 11.36 μg/mL.
Keywords ginger polysaccharide decolorization separation purification resin ultrafiltration
