蝙蝠蛾拟青霉固体中试发酵工艺研究_生物工程.doc

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摘要:本课题实验以一株蝙蝠蛾拟青霉为出发菌株,以培养物中所含有总核苷含量为指标对该蝙蝠蛾拟青霉菌株的固体发酵工艺进行了初步的工艺研究。结果显示蝙蝠蛾拟青霉最佳的固体培养基配方:75%的麦麸,5%的黄豆粉,20%的米糠;最佳的培养温度为24℃,培养基含水量(固液比)为1:1.3,接种量为30%。在上述的最佳培养基配方和最佳培养条件下培养的样品的总核苷含量为8.636±0.30mg/g,同时进行了多糖测定,结果表明在最佳培养工艺下样品的多糖含量为79.976±0.754mg/g。

关键词 蝙蝠蛾拟青霉;固体发酵;活性成分

 

Abstract:In the experiment,the starting strain of Paecilomyces Hepialidae to the total nucleotide content of culture as an indicator of the strain of the solid fermentation process was a preliminary study.The results showed that the best Hepialidae Paecilomyces solid culture medium with 75% wheat bran, soybean meal 5%, 20% of the rice bran, the best culture conditions for the temperature: 24 ℃, medium water content (solid-liquid ratio) of solid materials: water, 1:1.3, inoculum: 30%.In the optimum medium and cultured under optimal culture conditions of total nucleoside content of the sample is8.636±0.30mg / g. Polysaccharides and also carried out the determination of ergosterol, the results show that the best training in the process of polysaccharide centent was 79.976±0.754mg/g.

Keywords  Paecilomyces Hepialidae  Solid culture  Active ingredient