摘 要：本实验对玉扇（Haworthia truncata）进行组织培养，无菌繁殖，建立了玉扇叶片愈伤的快繁体系，为：(1)启动培养基: MS;(2)分化培养基: MS+6-BA 2mg·L-1+NAA 0.1mg·L-1;(3) 增殖培养基: MS+6-BA 1.5 mg·L-1+NAA 0.1mg·L-1;(4) 复壮与生根培养基: MS+ NAA 0.1mg·L-1。以上培养基均加入3%的蔗糖，0.7%的琼脂,调pH至 6.0。培养的温度为25℃,光照8 h·d- 1,光照度2000lx 。
关键词：愈伤组织培养；快速繁殖;玉扇；
Abstract：This article applies Haworthia truncate to tissue culture and sterile breeding.Finally we estiablish the rapid propagation systems of Haworthia truncate embryogenic callus. They are :(1)Actuating medium: MS;（2）The differentiation medium：MS+6-BA 2 mg·L-1+NAA 0.1 mg·L-1;(3) Proliferation medium: MS +6-BA 1.5mg·L-1+NAA 0.1 mg·L-1; (4)Rejuvenation and rooting culture medium: MS+ NAA 0.1 mg·L-1。All these mediums should add 3% sucrose、0. 7%agar and with the appropriate ph6.0.At the same time,the culture temperature should set to 25℃, illumination time 8 h.d- 1 and illumination intensity is 2000 lx.
Key words: callus culture；rapid propagation；Haworthia truncate
